质粒名称: | pHR CD19-empty CAR |
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出品公司: | 爱迪基因 |
目录编号: | 113015 |
存储实验室: | Ron Vale |
载体骨架基本信息 |
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载体名称: | pHR |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳动物表达,慢病毒 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | CD19 CAR-P intracellular GFP |
物种来源: | H. sapiens (human), Synthetic |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | GFP |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pHR CD19-empty CAR was a gift from Ron Vale (Addgene plasmid # 113015 ; http://n2t.net/addgene:113015 ; RRID:Addgene_113015) |
参考文献部分: | Chimeric antigen receptors that trigger phagocytosis. Morrissey MA, Williamson AP, Steinbach AM, Roberts EW, Kern N, Headley MB, Vale R. Elife. 2018 Jun 4;7. pii: 36688. doi: 10.7554/eLife.36688. 36688 [pii] PubMed 29862966 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR CD19-empty CAR 10700 bp ds-DNA circular SYN 22-AUG-2018 DEFINITION for production of lentivirus encoding the CD19 CAR-P GFP only. ACCESSION . VERSION . KEYWORDS pHR CD19-empty CAR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10700) AUTHORS Morrissey MA, Williamson AP, Steinbach AM, Roberts EW, Kern N, Headley MB, Vale R TITLE Chimeric antigen receptors that trigger phagocytosis. JOURNAL Elife. 2018 Jun 4;7. pii: 36688. doi: 10.7554/eLife.36688. PUBMED 29862966 REFERENCE 2 (bases 1 to 10700) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 22, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10700 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1..17) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2..18) /label=pBluescriptKS /note="For pBluescript vector" LTR 108..341 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(366..384) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(367..384) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(674..693) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(907..926) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1026..1048 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1086..1104) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1172..1276 /gene="bla" /label=AmpR promoter CDS 1277..2137 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1495..1514) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2308..2896 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2797..2816 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3050..3067 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(3137..3157) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3142..3471 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3322..3457 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3384..3403 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 4605..4670 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4800..4820 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5245..5379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5282..5301) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5336..5355 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 5547..6180 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 6227..6352 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6849..7082 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 7596..7713 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7866..8273 /label=SFFV promoter /note="spleen focus-forming virus long terminal repeat (LTR) promoter" regulatory 8307..8316 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9330..10049 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(9375..9396) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(9636..9655) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 9983..10004 /label=EGFP-C /note="EGFP, forward primer" misc_feature 10110..10698 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(10163..10183) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10581..10592) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta 61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg 361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg 421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag 481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc 541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc 601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac 901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga 3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg 3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta 3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg 3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg 3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg 3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc 3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca 3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg 3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct 4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag 4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc 4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag 4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg 4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc 4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct 4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt 4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg 4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg 4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt 4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct 4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct 4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc 4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt 4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg 4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta 4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt 5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg 5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca 5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt 5461 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt 5521 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga 5581 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac 5641 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt 5701 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt 5761 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg 5821 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata 5881 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg 5941 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg 6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 6241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 6301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 6361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 6421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 6481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 6541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 6601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 6661 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 6721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 6781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 6841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 6901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 6961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 7021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg 7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa 7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat 7741 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt 7801 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag 7861 ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca 7921 gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag 7981 cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc 8041 gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca 8101 tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac 8161 caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag 8221 ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc 8281 tggagctctc gagaattctc acgcgtgcca ccatggcctt accagtgacc gccttgctcc 8341 tgccgctggc cttgctgctc cacgccgcca ggccggacat ccagatgaca cagactacat 8401 cctccctgtc tgcctctctg ggagacagag tcaccatcag ttgcagggca agtcaggaca 8461 ttagtaaata tttaaattgg tatcagcaga aaccagatgg aactgttaaa ctcctgatct 8521 accatacatc aagattacac tcaggagtcc catcaaggtt cagtggcagt gggtctggaa 8581 cagattattc tctcaccatt agcaacctgg agcaagaaga tattgccact tacttttgcc 8641 aacagggtaa tacgcttccg tacacgttcg gaggggggac caagctggag atcacaggtg 8701 gcggtggctc gggcggtggt gggtcgggtg gcggcggatc tgaggtgaaa ctgcaggagt 8761 caggacctgg cctggtggcg ccctcacaga gcctgtccgt cacatgcact gtctcagggg 8821 tctcattacc cgactatggt gtaagctgga ttcgccagcc tccacgaaag ggtctggagt 8881 ggctgggagt aatatggggt agtgaaacca catactataa ttcagctctc aaatccagac 8941 tgaccatcat caaggacaac tccaagagcc aagttttctt aaaaatgaac agtctgcaaa 9001 ctgatgacac agccatttac tactgtgcca aacattatta ctacggtggt agctatgcta 9061 tggactactg gggccaagga acctcagtca ccgtctcctc aaccacgacg ccagcgccgc 9121 gaccaccaac accggcgccc accatcgcgt cgcagcccct gtccctgcgc ccagaggcgt 9181 gccggccagc ggcggggggc gcagtgcaca cgagggggct ggacttcgcc tgtgatatct 9241 acatctgggc gcccttggcc gggacttgtg gggtccttct cctgtcactg gttatcaccc 9301 tttactgcaa ccacaggaac cgaagacgta tggtgagcaa gggcgaggag ctgttcaccg 9361 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 9421 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 9481 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 9541 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 9601 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 9661 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 9721 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 9781 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 9841 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 9901 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 9961 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 10021 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagagtc gacctgcagg 10081 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 10141 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 10201 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 10261 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 10321 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 10381 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 10441 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 10501 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 10561 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 10621 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 10681 tttgggccgc ctccccgcat //
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